Prof. Dr. rer. nat. Donat Kögel
Experimental Neurosurgery
Frankfurt University Hospital
Theodor-Stern-Kai 7
D-60590 Frankfurt am Main
Tel ++49 69-6301-6923
Fax ++49 69- 6301-5575
E-Mail: koegel@em.uni-frankfurt.de
Scientific Focus
Mechanisms of programmed cell death, acute and chronic cns damage, neuronal stress signalling, physiological and pathophysiological roles of the amyloid precursor protein and its family members, mitochondrial control of cell death, target-specific therapies for the treatment of brain tumors, oncogenic signalling pathways, autophagy in health and disease, mechanisms of tumor cell migration and invasion.
Methods
Assays for detection of apoptosis (Caspase assays, FACS Annexin/propidium iodide, measurement of mitochondrial membrane potentials), autophagy and mitophagy (LC3 conversion and translocation, autophagic flux of GFP-LC3-RFP, FACS MTG, MTDR, Lysotracker) and cell viability (MTT assay), cultivation of primary neurons, organotypic cultures, in vitro hypoxia/ischemia models, reporter gene assays, shRNA/siRNA techniques, CRISPR/Cas, lentiviral gene transfer, live cell imaging, in vivo brain tumor models, in vitro models for cell migration and invasion, glioma stem-like cell models.
Selected Publications
Meyer N, Zielke S, Michaelis JB, Linder B, Warnsmann V, Rakel S, Osiewacz HD, Fulda S, Mittelbronn M, Münch C, Behrends C, Kögel D (2018). AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells. Autophagy, 14: 1693-1709.
Antonietti P, Linder B, Hehlgans S, Mildenberger IC, Burger MC, Fulda S, Steinbach JP, Gessler F, Rödel F, Mittelbronn M, Kögel D (2017). Interference with the HSF1/HSP70/BAG3 Pathway Primes Glioma Cells to Matrix Detachment and BH3 Mimetic-Induced Apoptosis. Mol Cancer Ther. 16:156-168.
Weissenberger J, Priester M, Bernreuther C, Rakel S, Glatzel M, Seifert V, Kögel, D (2010). Dietary Curcumin attenuates glioma growth in a syngeneic mouse model by inhibition of the JAK1, 2/STAT3 signaling pathway. Clin Cancer Res, 16: 5781-5795.
Kögel D, Schomburg R, Copanaki K, Prehn JHM. (2005). Regulation of Gene Expression by the Amyloid Precursor Protein: Inhibition of the JNK/c-Jun Pathway. Cell Death Differ. 12, 1-9.
Reimertz C, Kögel D, Rami A, Chittenden T, Prehn JH (2003). Gene Expression during ER Stress-Induced Apoptosis in Neurons: Induction of the BH3-Only Protein Bbc3/PUMA and Activation of the Mitochondrial Apoptosis Pathway. J. Cell Biol. 162: 587-597.