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Prof. Dr. rer. nat. Donat Kögel

Experimental Neurosurgery
Frankfurt University Hospital
Theodor-Stern-Kai 7
D-60590 Frankfurt am Main

Tel ++49 69-6301-6923
Fax ++49 69- 6301-5575

E-Mail: koegel@em.uni-frankfurt.de

Web

Scientific Focus

Mechanisms of programmed cell death, acute and chronic cns damage, neuronal stress signalling, physiological and pathophysiological roles of the amyloid precursor protein and its family members, mitochondrial control of cell death, target-specific therapies for the treatment of brain tumors, oncogenic signalling pathways, autophagy in health and disease, mechanisms of tumor cell migration and invasion.

Methods

Assays for detection of apoptosis (Caspase assays, FACS Annexin/propidium iodide, measurement of mitochondrial membrane potentials), autophagy and mitophagy (LC3 conversion and translocation, autophagic flux of GFP-LC3-RFP, FACS MTG, MTDR, Lysotracker) and cell viability (MTT assay), cultivation of primary neurons, organotypic cultures, in vitro hypoxia/ischemia models, reporter gene assays, shRNA/siRNA techniques, CRISPR/Cas, lentiviral gene transfer, live cell imaging, in vivo brain tumor models, in vitro models for cell migration and invasion, glioma stem-like cell models.

Selected Publications

Reisbeck L, Linder B, Tascher G, Bozkurt S, Weber KJ, Herold-Mende C, van Wijk SJL, Marschalek R, Schaefer L, Münch C, Kögel D. The iron chelator and OXPHOS inhibitor VLX600 induces mitophagy and an autophagy-dependent type of cell death in glioblastoma cells. Am J Physiol Cell Physiol. 2023 Dec 1;325(6):C1451-C1469. doi: 10.1152/ajpcell.00293.2023. Epub 2023 Oct 30. PMID: 37899749.

Haydo A, Wehle A, Herold-Mende C, Kögel D, Pampaloni F, Linder B. Combining organotypic tissue culture with light-sheet microscopy (OTCxLSFM) to study glioma invasion. EMBO Rep. 2023 Dec 6;24(12):e56964. doi: 10.15252/embr.202356964. Epub 2023 Nov 8. PMID: 37938214; PMCID: PMC10702825.

Meyer N, Henkel L, Linder B, Zielke S, Tascher G, Trautmann S, Geisslinger G, Münch C, Fulda S, Tegeder I, Kögel D. Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death. Autophagy. 2021 Nov;17(11):3424-3443. doi: 10.1080/15548627.2021.1874208. Epub 2021 Jan 19. PMID: 33461384; PMCID: PMC8632287.

Zielke S, Kardo S, Zein L, Mari M, Covarrubias-Pinto A, Kinzler MN, Meyer N, Stolz A, Fulda S, Reggiori F, Kögel D, van Wijk S. ATF4 links ER stress with reticulophagy in glioblastoma cells. Autophagy. 2021 Sep;17(9):2432-2448. doi: 10.1080/15548627.2020.1827780. Epub 2020 Oct 28. PMID: 33111629; PMCID: PMC8496713.

Meyer N, Zielke S, Michaelis JB, Linder B, Warnsmann V, Rakel S, Osiewacz HD, Fulda S, Mittelbronn M, Münch C, Behrends C, Kögel D. AT 101 induces early mitochondrial dysfunction and HMOX1 (heme oxygenase 1) to trigger mitophagic cell death in glioma cells. Autophagy. 2018;14(10):1693-1709. doi: 10.1080/15548627.2018.1476812. Epub 2018 Jul 21. PMID: 29938581; PMCID: PMC6135628.

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