Scientific Focus
Molecular and cellular biology of the mammalian circadian system; mechanisms of entrainment (synchronization) of the circadian rhythm generator in the hypothalamic suprachiasmatic nucleus and peripheral oscillators (hypophysis, adrenal gland).
Role of microglial cells and astrocytes in neurodegeneration and neuroprotection
Methods
Immunocytochemistry (qualitative, semiquantitative), Confocal Laser Scanning Microscopy, Electron Microscopy, Intravital Microscopy, Immunblotting, ELISA, RIA, In situ hybridization (radioactive, non-radioactive), Northern and Southern Blotting, PCR, RNAse Protection Assay; Microarrays, HPLC, Calcium Imaging, Cell and Organ Culture
Selected Publications
Kopp MDA, Schomerus C, Dehghani F, Korf HW, Meissl H (1999) Pituitary adenylate cyclase activating polypeptide and melatonin in the suprachiasmatic nucleus: effects on the calcium signal transduction cascade. J Neurosci 19: 206-219
Maronde E, Pfeffer M, Olcese J, Molina CA, Schlotter F, Dehghani F, Korf HW, Stehle J (1999) Transcription factors in neuroendocrine regulation: rhythmic changes in phosphoCREB and ICER levels frame melatonin synthesis. J Neurosci 19: 3326-3336
Kopp MDA, Meissl H, Dehghani F, Korf HW (2001) The pituitary adenylate cyclase-activating polypeptide modulates glutamatergic calcium signaling: investigations on rat suprachiasmatic nucleus neurons. J Neurochem 79: 161-171
von Gall C, Garabette ML, Kell CA, Frenzel S, Dehghani F, Schumm-Draeger PM, Weaver DR, Korf HW, Hastings MH, Stehle JH (2002) Rhythmic gene expression in pituitary depends on heterologous sensitization by the neurohormone melatonin. Nat Neurosci 3: 234-238
Gau D, Lemberger T, von Gall C, Kretz O, Le Minh N, Gass P, Schmid W, Schibler U, Korf HW, Schütz G (2002) Phosphorylation of CREB Ser142 regulates light-induced phase shifts of the circadian clock. Neuron 34: 245-253
Koch M, Mauhin V, Stehle JH, Schomerus C, Korf HW (2003) Dephosphorylation of pCREB by protein serine/threonine phosphatases is involved in inactivation of Aanat gene transcription in rat pineal gland. J Neurochem 85: 170-179
Arens J, Moar KM, Eiden S, Weide K, Schmidt I, Mercer JG, Simon E, Korf HW (2003)Age-dependent hypothalamic expression of neuropeptides in wildtype and melanocortin-4 receptor deficient mice. Physiol Genomics 16:38-46
Dehghani F, Conrad A, Kohl A, Korf HW, Hailer NP (2004) Clodronate inhibits the secretion of proinflammatory cytokines and NO by isolated microglial cells and reduces the number of proliferating glial cells in excitotoxically injured organotypic hippocampal slice cultures. Exp Neurol 189: 241-251
Korf HW, Wicht H (2004) The public cadaver. Nature 428: 805 (Invited concept assay)
Schomerus C, Laedtke E, Korf HW (2004) Activation of arylalkylamine N-acetyltransferase by phorbol esters in bovine pinealocytes suggests a novel regulatory pathway in melatonin synthesis. J Neuroendocrinol. 16:741-749
Gall C von, Weaver DR, Moek J, Jilg A, Stehle JH, Korf HW (2005)Melatonin plays a crucial role in the regulation of rhythmic clock gene expression in the mouse pars tuberalis. Ann N Y Acad Sci 1040 : 508-511
Schomerus C, Korf HW (2005) Mechanisms regulating melatonin synthesis in the mammalian pineal gland. Ann NY Acad Sci 1057: 372-383
Torres-Farfan C, Seron-Ferre M, Dinet V, Korf HW (2006)Immunocytochemical demonstration of day/night changes of clock gene protein levels in the murine adrenal gland: differences between melatonin-proficient (C3H) and melatonin-deficient (C57BL) mice. J Pineal Res 40:64-70
Korf HW, von Gall C (2006)
Mice, melatonin and the circadian system. Mol Cell Endocrinol. Apr 25 [Epub ahead of print]
Oest TM, Dehghani F, Korf HW, Hailer NP (2006)The immunosuppressant mycophenolate mofetil improves preservation of the perforant path in organotypic hippocampal slice cultures: a retrograde tracing study.
Hippocampus. 16(5) : 437-42.
Mishra SK, Braun N, Shukla V, Fullgrabe M, Schomerus C, Korf HW, Gachet C, Ikehara Y, Sevigny J, Robson SC, Zimmermann H (2006) Extracellular nucleotide signaling in adult neural stem cells: synergism with growth factor-mediated cellular proliferation. Development. 133(4): 675-84
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